11943 Kcnv2 Status: Cancelled
| Gene | Kcnv2 |
| Chromosome | 19 |
| Deletion Start | 27,322,754 |
| Deletion End | 27,333,923 |
| Deletion Size | 11,170 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,323,048 | 27,323,067 | ACTCCAGCCCAAATGTCCAC |
| TUP | 27,323,069 | 27,323,093 | CTGAAAGTGAACGTAGGCGGCCACA |
| TUR | 27,323,102 | 27,323,121 | GCCAGCTCACAGCATTCCAG |
| TDF | 27,333,003 | 27,333,025 | GGGAGCATAGGAGAGGTAGTCAG |
| TDP | 27,333,026 | 27,333,049 | CATCCTGGGTGCCTGACACAGTGC |
| TDR | 27,333,051 | 27,333,074 | TGCAGAAGTCAATACAAAGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | GAGACTGAACTGGCTAAGAG |
| SD | AAGCTAGATGCCATGAGAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
