11945 Dvl1 Status: Cancelled
| Gene | Dvl1 |
| Chromosome | 4 |
| Deletion Start | 155,847,738 |
| Deletion End | 155,858,369 |
| Deletion Size | 10,632 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,848,168 | 155,848,186 | GCCCAGGCTTTCTGACCAC |
| TUP | 155,848,188 | 155,848,210 | ATCCACTGGTGCTCCTGTGCCCA |
| TUR | 155,848,212 | 155,848,230 | GGCACCCAACATGGACCAG |
| TDF | 155,857,830 | 155,857,850 | CCCTAGCTTCTCGCCTCTCAG |
| TDP | 155,857,852 | 155,857,876 | TGTTCCTGGTATCTGCATGGCTGCC |
| TDR | 155,857,897 | 155,857,916 | TCAGGGCAGGACCTGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAGGCGGTTCGTGAACAG |
| SD | AGATGTCTCCTTACAAGACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
