11946 Xkrx Status: ES cell colonies screened / QC positives
| Gene | Xkrx |
| Chromosome | X |
| Deletion Start | 134,160,431 |
| Deletion End | 134,149,066 |
| Deletion Size | 11,366 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,161,311 | 134,161,335 | GATTGGACAATTCTTCCTGAGTTTG |
| TUP | 134,161,287 | 134,161,310 | TGTTGTGAAAACGGGATGGGACCA |
| TUR | 134,161,261 | 134,161,280 | CGAGAGCACAAGGTGACCAC |
| TDF | 134,151,129 | 134,151,147 | ACTTCCCGCCTCGTGATTC |
| TDP | 134,151,107 | 134,151,128 | TGGTGCTCTTCTCGGCCACCTT |
| TDR | 134,151,088 | 134,151,106 | GGGCACAGCCTTCAACTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTTGTCCAAGTTTCTCAGC |
| SD | TGCTGGTATCCAAACCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 60 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
