| Gene | Stra13 |
| Chromosome | 11 |
| Deletion Start | 120,713,716 |
| Deletion End | 120,711,246 |
| Deletion Size | 2,471 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,712,820 | 120,712,840 | CCTCCTGGCCCAACTAACAAG |
| TUP | 120,712,794 | 120,712,818 | TCACCTGTGCCTGTGTCCATTTCCA |
| TUR | 120,712,741 | 120,712,760 | GAGCGTCAGCCACAGAGAAG |
| TDF | 120,711,920 | 120,711,941 | ACAGACAGCCTTGTGAGGAATG |
| TDP | 120,711,892 | 120,711,918 | CACTGACTCTGCCCTCCTTCTAGGAAC |
| TDR | 120,711,859 | 120,711,880 | CCCTGAAGTGCAAATGGAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TACCCTAGTCTGGCCCTGTG |
| SD | AATGGCAAAGAGAAAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11949A-A2 | VGB6 | C57BL/6NTac | |
| 11949A-B4 | VGB6 | C57BL/6NTac | |
| 11949A-H12 | VGB6 | C57BL/6NTac | |
| 11949A-F10 | VGB6 | C57BL/6NTac | |
| 11949A-E5 | VGB6 | C57BL/6NTac | |
| 11949A-A7 | VGB6 | C57BL/6NTac | |
