11951 Xrcc2 Status: ES cell colonies screened / QC no positives
| Gene | Xrcc2 |
| Chromosome | 5 |
| Deletion Start | 25,703,524 |
| Deletion End | 25,689,902 |
| Deletion Size | 13,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,705,472 | 25,705,493 | GGTAGCTTGCTTCACCTCTAAC |
| TUP | 25,705,448 | 25,705,470 | AACCTGTTTGTGGCATCCGGCCT |
| TUR | 25,705,393 | 25,705,412 | AAATGGCTTGCGTGGCTCTG |
| TDF | 25,692,336 | 25,692,356 | AATGCAGAAAGGCTCAGACTC |
| TDP | 25,692,314 | 25,692,335 | TGCAGACGGGCCTTCTTCCTCC |
| TDR | 25,692,276 | 25,692,295 | TAGGCTCTGTAGCCCATGTC |
PCR Primers
| Primer | Sequence |
| SU | TTGCCTGATAAAGACCCGTC |
| SD | GCAGAAAGTGACCTAGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
