11955 Crct1 Status: ES cell colonies screened / QC no positives
| Gene | Crct1 |
| Chromosome | 3 |
| Deletion Start | 93,015,383 |
| Deletion End | 93,015,068 |
| Deletion Size | 316 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,014,779 | 93,014,800 | CCAAAGGGTCTTCCCAGAGTTC |
| TUP | 93,014,753 | 93,014,772 | CTGCCCCGCCCCGACTCCTG |
| TUR | 93,014,721 | 93,014,739 | GCCACAGCAAGAGGAGGAG |
| TDF | 93,014,636 | 93,014,654 | ACCGGCTGCTGCTGCTTTC |
| TDP | 93,014,616 | 93,014,635 | CCAGGAGACGCCGCCGACAG |
| TDR | 93,014,546 | 93,014,568 | AACTCTGATTGTTGCAGGAGCGT |
PCR Primers
| Primer | Sequence |
| SU | TGGTCCTAATGTCAGTCAGG |
| SD | CCATAGCGTGATCTGAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
