11967 Six5 Status: ES cell colonies screened / QC no positives
| Gene | Six5 |
| Chromosome | 7 |
| Deletion Start | 19,094,640 |
| Deletion End | 19,097,758 |
| Deletion Size | 3,119 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,095,153 | 19,095,173 | CTGGGCGCTGTGGACAAATAC |
| TUP | 19,095,174 | 19,095,197 | CGGCTGCGCAAGAAGTTCCCTCTG |
| TUR | 19,095,219 | 19,095,240 | TGAAGCAATAGACGGTCTCCTC |
| TDF | 19,097,470 | 19,097,489 | GGCCTGCTTTCCAGCTTCTC |
| TDP | 19,097,495 | 19,097,522 | CCCTACCTGAAGGTCTGATGTTGTCACC |
| TDR | 19,097,553 | 19,097,576 | CCTTCTACTCCTGTGCTCAGTTCC |
PCR Primers
| Primer | Sequence |
| SU | CCATTACCTTCTCCGTTGCTC |
| SD | CAGGGCCTTCTCACACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
