11969   Emd     Status: ES cell colonies screened / QC no positives

GeneEmd
ChromosomeX
Deletion Start74,254,955
Deletion End74,257,348
Deletion Size2,394
Genome Build38
MGI IDMGI:108117
NCBI Gene ID13726
BAC ID426P6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,255,57974,255,598GGATTTGGGCACAGGGAAAC
TUP74,255,59974,255,623CCCAGACCTCCCTCACCTCCTTGTA
TUR74,255,62574,255,646CCTCTGAAGCCACTGAGAACTG
TDF74,256,91474,256,937GGCAAGGATAGGTGAGTAGTGAAC
TDP74,256,94374,256,969TAGGGACAGGGACAAATTCGAGTCTCA
TDR74,256,97274,256,991GCAACAGGTAGGCAGGAGAG

PCR Primers

PrimerSequence
SUGCACGAGTTCTGAAAGCTC
SDTAAGGGTCAGAGGCAATAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR68 bp++-
TDFTDR78 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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