11971 Glipr2 Status: Cancelled
| Gene | Glipr2 |
| Chromosome | 4 |
| Deletion Start | 43,957,764 |
| Deletion End | 43,977,630 |
| Deletion Size | 19,867 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,958,645 | 43,958,664 | CCCACACCCTGTGTCATTTG |
| TUP | 43,958,668 | 43,958,696 | TCACTCCTGCATAGATAAGGGACAGGTGG |
| TUR | 43,958,716 | 43,958,734 | CCCACGAGGGAGGAAACTG |
| TDF | 43,977,537 | 43,977,557 | TCCTTTGTGGTGGCTAGATAC |
| TDP | 43,977,559 | 43,977,585 | TCCCAGCAGGGAATATTGTCAACCAGG |
| TDR | 43,977,594 | 43,977,614 | TGGAGGTGGGACATTCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCATTCGTATCCACATCTG |
| SD | TCTTACCCAATTGTGGTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
