11974   Rimklb     Status: ES cell colonies screened / QC positives

GeneRimklb
Chromosome6
Deletion Start122,479,908
Deletion End122,466,292
Deletion Size13,617
Genome Build38
MGI IDMGI:1918325
NCBI Gene ID108653
BAC ID27N8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,472,567122,472,588GGCTGTGGTGATGGATGAGATG
TUP122,472,542122,472,565TGCTGACGGTCGAGCAAGGAAATC
TUR122,472,486122,472,507GCTCTGTGAGGCAGTCCTTATG
TDF122,459,155122,459,176CGAGTCATTGTTGTGGGAGGTC
TDP122,459,129122,459,153TGTAGTTGGCACCATGCTACGCTGT
TDR122,459,096122,459,118GGAACAGTTGCTTTGCATCCTTC

PCR Primers

PrimerSequence
SUCTAAGCAGGGTTCCTCCAG
SDGCTTTTAAGGTGACAAAGGGA
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR103 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11974A-A8VGB6C57BL/6NTac
11974A-H1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image