11978   Prx     Status: ES cell colonies screened / QC no positives

GenePrx
Chromosome7
Deletion Start27,504,983
Deletion End27,519,839
Deletion Size14,857
Genome Build38
MGI IDMGI:108176
NCBI Gene ID19153
BAC ID453L19
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF27,505,38227,505,400CCAGCAGATGGGCTCAGTG
TUP27,505,40327,505,428TACAGGTGCTTGCTGCCAAGTCTGAT
TUR27,505,46027,505,480GATCCGGGTCCTCTCCTCTAC
TDF27,519,33227,519,352ACCAGGTAGTTGAGGGTGATG
TDP27,519,36027,519,384TGGGCACAAACTCAAGGTTCGGCTG
TDR27,519,39527,519,412CCTTGGCCTTTGCCAGAC

PCR Primers

PrimerSequence
SUGTGAGATTCCTGCTTACAGC
SDAAGTTCAAGCTGAGGTAACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR99 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image