| Gene | Tnni2 |
| Chromosome | 7 |
| Deletion Start | 142,442,494 |
| Deletion End | 142,444,345 |
| Deletion Size | 1,852 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,443,278 | 142,443,298 | TCACTCCCACCATTCTGTTTC |
| TUP | 142,443,303 | 142,443,327 | AGTGTGATGCTCCAGATAGCGGCCA |
| TUR | 142,443,336 | 142,443,354 | GGCGGCTTTCCTCTTTCTC |
| TDF | 142,443,565 | 142,443,586 | ACCCAGGAACTCTGCAAACAAC |
| TDP | 142,443,589 | 142,443,614 | CATGCGAAGATCGACGTGGCTGAAGA |
| TDR | 142,443,633 | 142,443,651 | GCTCTTCTGCACCTTCACC |
PCR Primers
| Primer | Sequence |
| SU | TCTCAAGCCAAAGGAGCAAG |
| SD | AAGAAGCTGATCCCAGACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11982A-A6 | VGB6 | C57BL/6NTac | |
| 11982A-A7 | VGB6 | C57BL/6NTac | |
| 11982A-B9 | VGB6 | C57BL/6NTac | |
| 11982A-G11 | VGB6 | C57BL/6NTac | |
| 11982A-F6 | VGB6 | C57BL/6NTac | |
| 11982A-E2 | VGB6 | C57BL/6NTac | |
