11983   Bhlhb9     Status: ES cell colonies screened / QC positives

GeneBhlhb9
ChromosomeX
Deletion Start135,889,044
Deletion End135,890,704
Deletion Size1,661
Genome Build38
MGI IDMGI:1917487
NCBI Gene ID70237
BAC ID383G14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,889,698135,889,717CCCAAAGCTCCTGCTGTAAC
TUP135,889,718135,889,747TCCAGCAGTGTTAGGTTATAGATCCCAGGA
TUR135,889,766135,889,789GAGGCCAGAACAATGTATGAAGAG
TDF135,889,913135,889,937CAAGATCAGAGAAGAGAATGGCATC
TDP135,889,939135,889,960AGCCCTTCGCTTGCCCTTGCAA
TDR135,889,962135,889,984GGAGAATCCAAATAGCACTCCAG

PCR Primers

PrimerSequence
SUTTCTCCACTGGTAGACTTGG
SDAACTAGATTACAAGAGCAGTAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR92 bp++-
TDFTDR72 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11983A-C2VGB6C57BL/6NTac
11983A-D9VGB6C57BL/6NTac
11983A-D10VGB6C57BL/6NTac
11983A-E10VGB6C57BL/6NTac
11983A-G4VGB6C57BL/6NTac
11983A-G9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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