| Gene | Nsun4 |
| Chromosome | 4 |
| Deletion Start | 116,053,773 |
| Deletion End | 116,033,983 |
| Deletion Size | 19,791 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,053,726 | 116,053,743 | CTGCTGAAGCTCGTGGAC |
| TUP | 116,053,701 | 116,053,722 | ACGCCGGTCCCGCGGAGATATC |
| TUR | 116,053,643 | 116,053,661 | CGCAAGGGCTGGATAAGTC |
| TDF | 116,034,464 | 116,034,486 | GGGTTGTAGCTCACAAAGGAAAG |
| TDP | 116,034,437 | 116,034,462 | ACCAAGCAAACATCCAGCAAGGCTCC |
| TDR | 116,034,403 | 116,034,423 | CCAGTGGAGTGGCAGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | GGATCCTAAGCGGCGTAGTC |
| SD | ACTCACCAGTGTGGGAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11984A-D11 | VGB6 | C57BL/6NTac | |
| 11984A-G7 | VGB6 | C57BL/6NTac | |
| 11984A-G8 | VGB6 | C57BL/6NTac | |
