11985   Armcx5     Status: ES cell colonies screened / QC positives

GeneArmcx5
ChromosomeX
Deletion Start135,745,180
Deletion End135,747,034
Deletion Size1,855
Genome Build38
MGI IDMGI:2148026
NCBI Gene ID494468
BAC ID267G14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,745,280135,745,303AGACCACTAAAGCCGTTAAGGCAG
TUP135,745,324135,745,349AACCAGGCTGAGGCTAGGGATGAAGC
TUR135,745,377135,745,397GTCACAGTCTTGGGCTCAGTG
TDF135,746,190135,746,214GAAGTATGGACCCAATCCAAATACC
TDP135,746,215135,746,238TGCCGCTGTAAATCCCGCACCTTT
TDR135,746,265135,746,287TTAACTTAAGTAGGGCAACGAGC

PCR Primers

PrimerSequence
SUTGACAGATCTGATGGCCTTG
SDTCTGCCAATTTGTGAACCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR118 bp++-
TDFTDR98 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11985A-E12VGB6C57BL/6NTac
11985A-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image