11992 Atp8b1 Status: ES cell colonies screened / QC no positives
| Gene | Atp8b1 |
| Chromosome | 18 |
| Deletion Start | 64,605,375 |
| Deletion End | 64,577,418 |
| Deletion Size | 27,958 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,605,087 | 64,605,108 | TCACATGGTCTAGGAGGGTATC |
| TUP | 64,605,061 | 64,605,085 | TCCTCCTTTGATGTCCTCCTCGGGT |
| TUR | 64,605,017 | 64,605,035 | TCCACCCTTGCCACATTGG |
| TDF | 64,578,004 | 64,578,024 | GACCATGTTGGGAGCTTTACC |
| TDP | 64,577,975 | 64,578,002 | AATCAGTTTAGCCGGCCATATTGCCATC |
| TDR | 64,577,930 | 64,577,951 | GATGTGTTCAGCCAGTGGTTAC |
PCR Primers
| Primer | Sequence |
| SU | TCCCTCAGGTTCTTCTGCTC |
| SD | GAAGGCGAGGTGAATCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
