11994   Minpp1     Status: ES cell colonies screened / QC positives

GeneMinpp1
Chromosome19
Deletion Start32,485,944
Deletion End32,514,375
Deletion Size28,432
Genome Build38
MGI IDMGI:1336159
NCBI Gene ID17330
BAC ID258O16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 19

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF32,486,74532,486,762GCGCAAGAGTGCAGTAGG
TUP32,486,76532,486,788ACGCTGGAGACCCAGCTAGAAGGA
TUR32,486,81832,486,839CGCGATTTGGGAATGTGGAATG
TDF32,514,25332,514,273AGACCCGTCGGGTTGTATGAG
TDP32,514,27532,514,298AGCTCAAGACCCACTACCGGGACA
TDR32,514,33932,514,358CGGACGTGATGTTCGCTTTG

PCR Primers

PrimerSequence
SUCTTTGAATCGGTTCCTGAGG
SDAAATCTCAGCTCTGCAAACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR95 bp++-
TDFTDR106 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11994A-F3VGB6C57BL/6NTac
11994A-B2VGB6C57BL/6NTac