12000 B3gnt4 Status: Cancelled
| Gene | B3gnt4 |
| Chromosome | 5 |
| Deletion Start | 123,510,577 |
| Deletion End | 123,511,645 |
| Deletion Size | 1,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,510,776 | 123,510,798 | GCCTCTTCTTAACCTATCGACAC |
| TUP | 123,510,799 | 123,510,824 | TGCCGAAACTTCTCCATCTTGCTGGA |
| TUR | 123,510,864 | 123,510,884 | TGAGCAGGCTGTGACTTGATG |
| TDF | 123,511,221 | 123,511,242 | AGTGGGAGATGTCATTCGCTTG |
| TDP | 123,511,245 | 123,511,266 | CCGGCCCAACAGGAACACCAAA |
| TDR | 123,511,305 | 123,511,326 | TCCTGCATAAGGTGGGTAGTGG |
PCR Primers
| Primer | Sequence |
| SU | GATAGCTACAAGCCACATGG |
| SD | GTGAGTCTTCAGGCTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
