| Gene | Pih1d1 |
| Chromosome | 7 |
| Deletion Start | 45,156,313 |
| Deletion End | 45,159,993 |
| Deletion Size | 3,681 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,156,468 | 45,156,488 | GTGGCTTCAGTTCCCAGAGTG |
| TUP | 45,156,490 | 45,156,510 | CCCATGGCAGCAGTGAGGCCT |
| TUR | 45,156,523 | 45,156,543 | AGGAAACCTGAGGCAAACAAC |
| TDF | 45,159,294 | 45,159,314 | ACCTGCTCTTGGCTGAAGTTG |
| TDP | 45,159,315 | 45,159,340 | ACCTTCCTAAACTGGTGAGTGCAGGC |
| TDR | 45,159,347 | 45,159,366 | CACTGGCTCTTCTCCCTGAC |
PCR Primers
| Primer | Sequence |
| SU | AGTTCCTTAGTTATACAGACG |
| SD | AAGTAGAGTCAGCTCTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12006A-A4 | VGB6 | C57BL/6NTac | |
| 12006A-B6 | VGB6 | C57BL/6NTac | |
| 12006A-F5 | VGB6 | C57BL/6NTac | |
| 12006A-F2 | VGB6 | C57BL/6NTac | |
