12009 Napepld Status: ES cell colonies screened / QC no positives
| Gene | Napepld |
| Chromosome | 5 |
| Deletion Start | 21,699,116 |
| Deletion End | 21,680,849 |
| Deletion Size | 18,268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,683,205 | 21,683,224 | TCCATCCCGAATGTGCTCAG |
| TUP | 21,683,172 | 21,683,199 | TGATAATGGAGAAGAATCACAGCGGCGT |
| TUR | 21,683,145 | 21,683,162 | GTGCTGCCCACCTCTTTG |
| TDF | 21,665,357 | 21,665,378 | TTGCCATCACTTCCAGCATTAC |
| TDP | 21,665,326 | 21,665,352 | AGCCGCCAGTGAAACTGAATGAAGCTC |
| TDR | 21,665,266 | 21,665,287 | ATCTCGACTCTCCATGCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TGTAACACGCGAGCCATTTG |
| SD | ACTCTCTGTAGAAAGCCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
