12010 Penk Status: ES cell colonies screened / QC no positives
| Gene | Penk |
| Chromosome | 4 |
| Deletion Start | 4,138,538 |
| Deletion End | 4,134,185 |
| Deletion Size | 4,354 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,137,764 | 4,137,784 | GGAACCCCAAGATGTGTTCTC |
| TUP | 4,137,733 | 4,137,762 | TCATTACCTCGGATAGCACTTCATCTAGGC |
| TUR | 4,137,697 | 4,137,720 | TGAAGGGTTTGGGTGTTAAAGTTG |
| TDF | 4,134,200 | 4,134,220 | GGATGCAGATGAGGGAGACAC |
| TDP | 4,134,176 | 4,134,197 | TGGCCAACTCCTCCGATCTGCT |
| TDR | 4,134,155 | 4,134,175 | CCCGTTCCCAGTAGCTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AAGGACAGGATGTCATCAGG |
| SD | CACAGACAAATGACAGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
