12019 Dvl2 Status: ES cell colonies screened / QC one positive
| Gene | Dvl2 |
| Chromosome | 11 |
| Deletion Start | 70,000,836 |
| Deletion End | 70,009,627 |
| Deletion Size | 8,792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,001,068 | 70,001,090 | AGAGTGGATTGGGCGATATTGAG |
| TUP | 70,001,095 | 70,001,121 | ACCGGTCTACCTTGGACTGTTTCTGGC |
| TUR | 70,001,147 | 70,001,167 | GGGCTTTCTACCTTAGCACAG |
| TDF | 70,008,980 | 70,009,000 | AGCCAGCACAGTGAGGGTAAG |
| TDP | 70,009,001 | 70,009,026 | TGACCCACTCCCACGTACCAGTACAG |
| TDR | 70,009,032 | 70,009,052 | GCTCTACTTGTGCCCACTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGACTCAAGGAAGTCACGTG |
| SD | AGAGCTGACTAGGCACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
