12022 Mat1a Status: Cancelled
| Gene | Mat1a |
| Chromosome | 14 |
| Deletion Start | 41,105,594 |
| Deletion End | 41,122,610 |
| Deletion Size | 17,017 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,106,365 | 41,106,388 | AGTACAGAGGCATCACATCACAAG |
| TUP | 41,106,390 | 41,106,412 | CTCAATGACGCCAAGGGCTGGGA |
| TUR | 41,106,413 | 41,106,436 | CAAATTGGACACGATGGGAGTAAG |
| TDF | 41,121,789 | 41,121,807 | GCCATTGGTGTGGCAGAAC |
| TDP | 41,121,811 | 41,121,837 | TGTCCATTTCCATCTTCACCTACGGGA |
| TDR | 41,121,842 | 41,121,863 | CAGCTCCCGCTCAGTCTTATTG |
PCR Primers
| Primer | Sequence |
| SU | TCCTCCACTGTCCTTGCTTG |
| SD | AGGACTTCCTGTCAAACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
