12024 Rusc2 Status: ES cell colonies screened / QC no positives
| Gene | Rusc2 |
| Chromosome | 4 |
| Deletion Start | 43,414,699 |
| Deletion End | 43,426,692 |
| Deletion Size | 11,994 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,415,040 | 43,415,059 | AGGTGTGGGTGAGACAGGAC |
| TUP | 43,415,060 | 43,415,080 | TCGGTGACCTGCATGACAGCA |
| TUR | 43,415,095 | 43,415,113 | GGAAGGACTGCTGGGTGAC |
| TDF | 43,426,376 | 43,426,396 | GGGAATTGAGTGTTGGGAGAC |
| TDP | 43,426,400 | 43,426,425 | ACTGGCTGTTTACAGGGCTTCTCCGA |
| TDR | 43,426,438 | 43,426,456 | TGCACCTCGCTGGAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TACTGGACCTGGATACACAG |
| SD | CCTACTCTGACGACCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
