12027 Tomm6 Status: Cancelled
| Gene | Tomm6 |
| Chromosome | 17 |
| Deletion Start | 47,687,637 |
| Deletion End | 47,686,785 |
| Deletion Size | 853 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,687,716 | 47,687,736 | CGGGCTACAGCGATAGTTCAG |
| TUP | 47,687,690 | 47,687,714 | CGCCAAGCCTGAGGATTTGGGATCC |
| TUR | 47,687,652 | 47,687,670 | CTTGTACCCTGCGGGTCTC |
| TDF | 47,687,360 | 47,687,380 | AAGGTGGTGCTGAGAGGAAAC |
| TDP | 47,687,329 | 47,687,358 | TGCCATAGTATGTCATTAGCTGCATGAGAC |
| TDR | 47,687,256 | 47,687,278 | GCAGCAAAGAGTCCCAAATTGAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTCTGACCTGAAAGACAC |
| SD | GTCAACTTCAGCTGCCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
