| Gene | Cutc |
| Chromosome | 19 |
| Deletion Start | 43,753,166 |
| Deletion End | 43,768,293 |
| Deletion Size | 15,128 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,753,700 | 43,753,721 | CCTGTCTCAGCGTGTTTAAGTG |
| TUP | 43,753,723 | 43,753,747 | TCTAAAGCTGCAGTCCGCTCCCAAG |
| TUR | 43,753,778 | 43,753,802 | TGCTCAAAGTTAGAGACTCCAGATG |
| TDF | 43,768,042 | 43,768,062 | TGGTAGTGCAGCATCCTTGAC |
| TDP | 43,768,066 | 43,768,095 | CCCTTACTCTGAGCCATGAATTAATGAGTC |
| TDR | 43,768,098 | 43,768,118 | TCCTACTGGCACTTCCATCTC |
PCR Primers
| Primer | Sequence |
| SU | AGCAGGAGTGGGTACCTGTG |
| SD | GCCCAGGGAGTACATGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12029B-B8 | VGB6 | C57BL/6NTac | |
| 12029B-C2 | VGB6 | C57BL/6NTac | |
| 12029B-D6 | VGB6 | C57BL/6NTac | |
| 12029B-F3 | VGB6 | C57BL/6NTac | |
