12033 Pla2g2e Status: Cancelled
| Gene | Pla2g2e |
| Chromosome | 4 |
| Deletion Start | 138,879,460 |
| Deletion End | 138,882,629 |
| Deletion Size | 3,170 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,880,160 | 138,880,182 | GATGGAAGCACAAAGCATGTTAC |
| TUP | 138,880,190 | 138,880,218 | CCTTGTTATTGATAGCTGGGTGCTTTGCA |
| TUR | 138,880,234 | 138,880,256 | TCCAGACATTTCCCCAAGGATTC |
| TDF | 138,882,004 | 138,882,025 | GCAGGAGGAAGTTGAGAGGTTG |
| TDP | 138,882,026 | 138,882,050 | TGAAGTCTTGGTACAGCCCTGGCAG |
| TDR | 138,882,065 | 138,882,084 | CCTGCCAGAGGCTTCGTAAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGTCATGAAGAGGGACG |
| SD | ACAAAGGACTTATGGCTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
