| Gene | A130010J15Rik |
| Chromosome | 1 |
| Deletion Start | 193,174,345 |
| Deletion End | 193,175,185 |
| Deletion Size | 841 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 193,174,633 | 193,174,652 | TTGGAACAGACACTGCTTGG |
| TUP | 193,174,655 | 193,174,681 | CTGTAGCTTTCGTGGACGTTTCCCGCT |
| TUR | 193,174,688 | 193,174,707 | CGACCGGACAGAAGGATGAG |
| TDF | 193,174,793 | 193,174,814 | TCTCCTACACCAGGCTACATTC |
| TDP | 193,174,817 | 193,174,846 | CAGACTGGAATCTCTGCACTGTATTTGGGA |
| TDR | 193,174,881 | 193,174,902 | GTTGTCATCTTCACGGTTCAGG |
PCR Primers
| Primer | Sequence |
| SU | GTAAGGTTTGAATCCCAGAGC |
| SD | CCTTAAGCATCATTGTCTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12040A-B9 | VGB6 | C57BL/6NTac | |
| 12040A-D10 | VGB6 | C57BL/6NTac | |
| 12040A-D7 | VGB6 | C57BL/6NTac | |
