12044 Rps20 Status: ES cell colonies screened / QC no positives
| Gene | Rps20 |
| Chromosome | 4 |
| Deletion Start | 3,835,483 |
| Deletion End | 3,834,421 |
| Deletion Size | 1,063 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,835,292 | 3,835,310 | CCCGCCTGGGTGATAACAG |
| TUP | 3,835,261 | 3,835,286 | TAAAGATACCGGAAAGACGCCCGTGG |
| TUR | 3,835,189 | 3,835,208 | GACCCTTCTCCAGCGACTTC |
| TDF | 3,834,794 | 3,834,815 | CAAATGTCTGGTGGCGATTGAG |
| TDP | 3,834,772 | 3,834,792 | ATGATACCTTTCTTAAAGCAA |
| TDR | 3,834,738 | 3,834,761 | GGTTTCAGCAACCTTTACTAGAGA |
PCR Primers
| Primer | Sequence |
| SU | CTGTCCTCCGAGTCAGGAC |
| SD | CCATTTCAAATAACTCAGAATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
