12048 Rps15a Status: ES cell colonies screened / QC no positives
| Gene | Rps15a |
| Chromosome | 7 |
| Deletion Start | 118,115,238 |
| Deletion End | 118,109,030 |
| Deletion Size | 6,209 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,114,811 | 118,114,832 | CACAGAGCTGGGAAGATTGTTG |
| TUP | 118,114,782 | 118,114,810 | TGAACCTCACAGGAAGGTTGAACAAGGTA |
| TUR | 118,114,758 | 118,114,780 | TGCAATTTGAGAAGCAAGATGTC |
| TDF | 118,109,956 | 118,109,979 | GACCTAGAGAAATGGCAGAACAAC |
| TDP | 118,109,931 | 118,109,954 | TGCTCCCTTCACGTCAGTTTGGGT |
| TDR | 118,109,868 | 118,109,888 | CAAGCCCAGCTATATCCCAAC |
PCR Primers
| Primer | Sequence |
| SU | TATCTTCTGTGTGGTGAGGC |
| SD | GAATAACCTGCGAACCCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
