12050 Calcoco2 Status: ES cell colonies screened / QC positives
| Gene | Calcoco2 |
| Chromosome | 11 |
| Deletion Start | 95,968,906 |
| Deletion End | 95,961,227 |
| Deletion Size | 7,680 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,967,976 | 95,967,999 | TGCAAGCAGGACTTAGTAAGATGG |
| TUP | 95,967,950 | 95,967,974 | TGAACCTTAGAGTTTTGATAGCTTT |
| TUR | 95,967,922 | 95,967,944 | AGGAGATCCTGTCTGGACCCTTC |
| TDF | 95,962,205 | 95,962,224 | AGCTGTCACCGTGGACACTG |
| TDP | 95,962,177 | 95,962,202 | CACTAGAAGCAGAGCCTGCAAGAGCA |
| TDR | 95,962,140 | 95,962,162 | CAACAGGCATCCAAGGACTAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGTGTGGGAAGGCAGTGG |
| SD | TGAGTCCTTTTGAGACAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12050A-C2 | VGB6 | C57BL/6NTac | |
| 12050A-D4 | VGB6 | C57BL/6NTac | |
| 12050A-F7 | VGB6 | C57BL/6NTac | |
| 12050A-E5 | VGB6 | C57BL/6NTac | |
