12052 Tmem45a Status: ES cell colonies screened / QC no positives
| Gene | Tmem45a |
| Chromosome | 16 |
| Deletion Start | 56,825,852 |
| Deletion End | 56,806,596 |
| Deletion Size | 19,257 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,825,731 | 56,825,752 | CTCGAACCTGCTACCTTAACTC |
| TUP | 56,825,694 | 56,825,721 | ATTACGTCGGACAGAGATTTGGGAAGGA |
| TUR | 56,825,647 | 56,825,670 | CCATGAATGGTCTCCTTACCAGTG |
| TDF | 56,807,423 | 56,807,446 | GCCAATGTTCTAGGTTAGAGTCAC |
| TDP | 56,807,397 | 56,807,421 | CCTGACTCTGGTTAGGGTTAGGCCA |
| TDR | 56,807,366 | 56,807,388 | GAGCTTCATCCATCGACTTCATC |
PCR Primers
| Primer | Sequence |
| SU | TGCTCAAAGGGCAAGTATATG |
| SD | CTGTATCATGTGAGGGTGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
