12054 Ttll10 Status: Cancelled
| Gene | Ttll10 |
| Chromosome | 4 |
| Deletion Start | 156,048,622 |
| Deletion End | 156,034,842 |
| Deletion Size | 13,781 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 156,048,328 | 156,048,349 | GTCAGCTCCAAGAGATCCAAAC |
| TUP | 156,048,303 | 156,048,325 | TCCAGAATCCATCCGTGCCACAC |
| TUR | 156,048,251 | 156,048,270 | GAGGGCATGTGTAACCAGTC |
| TDF | 156,035,389 | 156,035,406 | TGCTGCCTCTGCTGTCAC |
| TDP | 156,035,360 | 156,035,386 | CGTCGCTTTGTGCTCCTTTACAACGGG |
| TDR | 156,035,340 | 156,035,357 | GGCCACAAGTCCGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | TTCCAAAGACGGCAAATAGG |
| SD | GTGTCTGAAAGGCCAGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
