12055 Itm2a Status: ES cell colonies screened / QC one positive
| Gene | Itm2a |
| Chromosome | X |
| Deletion Start | 107,403,213 |
| Deletion End | 107,397,851 |
| Deletion Size | 5,363 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,403,140 | 107,403,161 | AAGATGTAGAGGCGCTCGTCAG |
| TUP | 107,403,117 | 107,403,139 | TCGCACTGTCCGAGCTCAAATCC |
| TUR | 107,403,095 | 107,403,115 | GTGGGAACTACCTTGCCAGTC |
| TDF | 107,398,114 | 107,398,137 | ACCGAAAATCCTTCCGCCTTAGAC |
| TDP | 107,398,089 | 107,398,112 | CAGAGACCTTCTGCTGGGTAGGCA |
| TDR | 107,398,009 | 107,398,029 | TGTGAGTTTGCTGTGCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | GGTGCAGAAGGCAGTAGGAG |
| SD | GCTCTAGCCAAGTGATGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 129 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
