12062 Arl6ip4 Status: Cancelled
| Gene | Arl6ip4 |
| Chromosome | 5 |
| Deletion Start | 124,116,449 |
| Deletion End | 124,118,091 |
| Deletion Size | 1,643 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,116,698 | 124,116,718 | CGAGGGTGTGAAGAGGTTCTG |
| TUP | 124,116,719 | 124,116,746 | TTGAGTGGAGGGTGTGCCTTATACATGC |
| TUR | 124,116,747 | 124,116,768 | TCGAAGACCCAAAGCATTCAAG |
| TDF | 124,117,614 | 124,117,635 | GTTAATGGTGCAGGCTGACAAG |
| TDP | 124,117,638 | 124,117,662 | AGAGCCTACCTGGCCCGTCTCCATT |
| TDR | 124,117,677 | 124,117,697 | GCGCTCCTCCCAATCAGTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTAGAGGCGGAACCCAGAG |
| SD | GTATGGGACAGGAGACAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
