| Gene | Spag4 |
| Chromosome | 2 |
| Deletion Start | 156,065,361 |
| Deletion End | 156,069,475 |
| Deletion Size | 4,115 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 156,066,215 | 156,066,234 | CGAGGGTGTCCAAGAGCTTC |
| TUP | 156,066,235 | 156,066,256 | CTGAGTACGAGCCAGGCCAGCC |
| TUR | 156,066,310 | 156,066,328 | GCATCTGGGCTGGTTTCTC |
| TDF | 156,069,034 | 156,069,056 | TACCATGAGAACCTAAGCCTGAG |
| TDP | 156,069,059 | 156,069,086 | CGCATACTGTGGAATAGTTGGCTTTCCC |
| TDR | 156,069,097 | 156,069,117 | GAAGAACAGTGAGGCCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | TGGCTCCCAGTGACGTCA |
| SD | TCACTCGTACTTGACTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12063A-A5 | VGB6 | C57BL/6NTac | |
| 12063A-B3 | VGB6 | C57BL/6NTac | |
| 12063A-D9 | VGB6 | C57BL/6NTac | |
| 12063A-G12 | VGB6 | C57BL/6NTac | |
