12064 Dach2 Status: Cancelled
| Gene | Dach2 |
| Chromosome | X |
| Deletion Start | 113,298,430 |
| Deletion End | 113,298,932 |
| Deletion Size | 503 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,298,590 | 113,298,607 | TCTTGGGTGGCGGGATTG |
| TUP | 113,298,615 | 113,298,635 | CAGCGGTGGCAGCAGCAACAC |
| TUR | 113,298,636 | 113,298,654 | CCGGCACTCGTTTGTGTTG |
| TDF | 113,298,716 | 113,298,733 | GCCTGCCGCAAGTCTTTG |
| TDP | 113,298,738 | 113,298,761 | TTTCCTCAAGCACCTGGTGGGAGG |
| TDR | 113,298,777 | 113,298,799 | TGTCGAGTCTCTTCAGCTTAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGGTAGGCAGAGGCTG |
| SD | CCCTACCACTGAAAGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
