12066 Cggbp1 Status: ES cell colonies screened / QC no positives
| Gene | Cggbp1 |
| Chromosome | 16 |
| Deletion Start | 64,855,576 |
| Deletion End | 64,856,077 |
| Deletion Size | 502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,855,719 | 64,855,739 | GCTGAATCATGTTCGCAAGTC |
| TUP | 64,855,740 | 64,855,768 | TGCCATTAGTGACCACCTCAAGTCAAAGA |
| TUR | 64,855,807 | 64,855,827 | GGGCCTCTGCTTCTTTCTCAC |
| TDF | 64,855,959 | 64,855,977 | CCTGTCCCGGCATGTGAAG |
| TDP | 64,855,980 | 64,856,006 | CGGAGGGTCCATACCTAAGTCAGACCA |
| TDR | 64,856,008 | 64,856,027 | GGCAGATATGCTCGCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CAAGGTGTGCACATAGGCAG |
| SD | TTTCCGGCCTACTATTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
