12072 Atp5c1 Status: ES cell colonies screened / QC no positives
| Gene | Atp5c1 |
| Chromosome | 2 |
| Deletion Start | 10,056,789 |
| Deletion End | 10,080,400 |
| Deletion Size | 23,612 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,080,088 | 10,080,107 | TTTGGAGCCATCTGGGTTTC |
| TUP | 10,080,060 | 10,080,085 | TCAACTCCGTACTTCCGCTCCGTACC |
| TUR | 10,080,014 | 10,080,033 | CTTCACCTTCAGGGCCTTTC |
| TDF | 10,057,623 | 10,057,645 | CCTGGTCCTAAACTCTTGCTTTC |
| TDP | 10,057,598 | 10,057,622 | TGAACATAGGACTGCCCATCTCCCA |
| TDR | 10,057,565 | 10,057,587 | GCTGTGATGCTAACAGGGTAGTG |
PCR Primers
| Primer | Sequence |
| SU | ATCTGGCAGTACCAGCGAAG |
| SD | TGTGAGCCAGACATCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
