| Gene | Paip2b |
| Chromosome | 6 |
| Deletion Start | 83,831,296 |
| Deletion End | 83,808,821 |
| Deletion Size | 22,476 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,831,091 | 83,831,111 | TCTGTTACGTGGAACGGGATG |
| TUP | 83,831,063 | 83,831,089 | AACGGGATTCTGACCCAGCTTAGAGCA |
| TUR | 83,831,038 | 83,831,062 | GGCCCTTCCTCATAGTGTAAATTAG |
| TDF | 83,809,096 | 83,809,117 | GGGCTGGACTTTGAGTATTGAC |
| TDP | 83,809,064 | 83,809,093 | TGCTAGGCTTGTACTCATCAGTGAGCTACA |
| TDR | 83,808,968 | 83,808,990 | GGCAGGTGGATCTCTGTGATTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGGCTCGCGCTCTTAGG |
| SD | AAGTTCTCCAGGAACCCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12080A-H3 | VGB6 | C57BL/6NTac | |
