12081 C1galt1c1 Status: ES cell colonies screened / QC positives
| Gene | C1galt1c1 |
| Chromosome | X |
| Deletion Start | 35,985,290 |
| Deletion End | 35,984,244 |
| Deletion Size | 1,047 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCCACATTAGGATTGGAAACAG |
| TUP | | | TGCACCACCATGAGCATCACCATC |
| TUR | | | CTCCATGCGTTCAGCCTCTG |
| TDF | | | TGTTGCTCTGATATGGCTGTTAC |
| TDP | | | TGGACTGACTCCTAATCAGATGCACGTGA |
| TDR | | | CAAATGCCCTAAGCCGGTACAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCAATCCTTGAATGAGTTTC |
| SD | ACTTTCTCAAGCATGGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
