12087 2810002N01Rik Status: ES cell colonies screened / QC no positives
| Gene | 2810002N01Rik |
| Chromosome | 12 |
| Deletion Start | 111,713,296 |
| Deletion End | 111,733,815 |
| Deletion Size | 20,520 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CGTTACGGATGGCTGTGAACTAC |
| TUP | | | CATGTGGTTGCTGGGATTTGAACTCTGGA |
| TUR | | | GGGCACCTGACTGCTCTTTG |
| TDF | | | TCAGGAAGGTAGATGGAGTTTGTG |
| TDP | | | CCTACACTGTGGCTGACTTGTGCTGTC |
| TDR | | | CAGAAGGTAAGAGAGGAGCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCGCCCCACGCTCACCTG |
| SD | ACCACCAGAGCCAGCTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
