| Gene | Dmap1 |
| Chromosome | 4 |
| Deletion Start | 117,681,934 |
| Deletion End | 117,674,698 |
| Deletion Size | 7,237 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,681,550 | 117,681,569 | TCTTGAGGTGAGGGAAGGAC |
| TUP | 117,681,521 | 117,681,545 | TTCCCTGATAACTCAGACGCGAGCG |
| TUR | 117,681,478 | 117,681,497 | TCATGGGAGCGTCTCTACAC |
| TDF | 117,674,778 | 117,674,797 | TAGCGGAAACGACGGGAATC |
| TDP | 117,674,746 | 117,674,773 | TCCAGCTCATCTTCTGTGAAGAAAGCCA |
| TDR | 117,674,707 | 117,674,729 | ACACCATACCACCAACTCAGATG |
PCR Primers
| Primer | Sequence |
| SU | CGGCTCCTGTTTGTCTAAGC |
| SD | GATATGCCAATGTTCCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12088A-F2 | VGB6 | C57BL/6NTac | |
| 12088A-C4 | VGB6 | C57BL/6NTac | |
| 12088A-C11 | VGB6 | C57BL/6NTac | |
| 12088A-D9 | VGB6 | C57BL/6NTac | |
| 12088A-D11 | VGB6 | C57BL/6NTac | |
| 12088A-F12 | VGB6 | C57BL/6NTac | |
