12089 Eno2 Status: Cancelled
| Gene | Eno2 |
| Chromosome | 6 |
| Deletion Start | 124,768,319 |
| Deletion End | 124,761,207 |
| Deletion Size | 7,113 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,768,078 | 124,768,102 | GCTCGGTGAGTCTATGTTCTAGTTC |
| TUP | 124,768,055 | 124,768,077 | AGCGTGAGAAAGGCCCTGCCAGT |
| TUR | 124,768,003 | 124,768,023 | GGCCTCAGAAACAGGGCTATG |
| TDF | 124,761,574 | 124,761,592 | TAGGCCACCATTCCACCTC |
| TDP | 124,761,542 | 124,761,568 | TCCAGGACCTGGATAGCAGTTGATGCC |
| TDR | 124,761,474 | 124,761,494 | GGCTTTCGTGGTCAGTTAGTG |
PCR Primers
| Primer | Sequence |
| SU | CGGTGTTCTCCCATCTTGTC |
| SD | AAGCCTCAACCAAGCATGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
