12093 Zmym3 Status: ES cell colonies screened / QC no positives
| Gene | Zmym3 |
| Chromosome | X |
| Deletion Start | 101,418,936 |
| Deletion End | 101,405,575 |
| Deletion Size | 13,362 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,418,755 | 101,418,775 | AGCCCTGGACCTGCTTGATAC |
| TUP | 101,418,729 | 101,418,751 | TTCTGGCCTGGAGAAGGACCCTG |
| TUR | 101,418,705 | 101,418,725 | CAGTGGCTCCATCCAGAACTC |
| TDF | 101,406,476 | 101,406,498 | GAGTTCTGACCTGTCTGTGAGTG |
| TDP | 101,406,450 | 101,406,474 | TTTGCCAAGTCCCTTCTGTGGGCCT |
| TDR | 101,406,399 | 101,406,419 | GGGCTTGCCTTCCAAATGATG |
PCR Primers
| Primer | Sequence |
| SU | AGGGAGGAAGGAGCTACGAG |
| SD | GCTGGGTTCTTCCCTAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
