12096 Daglb Status: ES cell colonies screened / QC no positives
| Gene | Daglb |
| Chromosome | 5 |
| Deletion Start | 143,464,685 |
| Deletion End | 143,503,410 |
| Deletion Size | 38,726 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,465,560 | 143,465,578 | AGCATCTGCGGAGAGCATC |
| TUP | 143,465,579 | 143,465,607 | TGCGGTTCACAGTCATCCTTGGATATACA |
| TUR | 143,465,621 | 143,465,640 | GGGAGGGTCTTGTGCAACTC |
| TDF | 143,502,867 | 143,502,887 | GATCTTGCTGCATGGCTGTTG |
| TDP | 143,502,889 | 143,502,911 | TACGGACTGTTCGGAGGAAGCCC |
| TDR | 143,502,923 | 143,502,943 | TGCCCTCATCCAGTTCAGTTG |
PCR Primers
| Primer | Sequence |
| SU | ACCGGAATGGACTTCATACG |
| SD | CTATGCGTGTTCCCAACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
