12100 1700014N06Rik Status: ES cell colonies screened / QC positives
| Gene | 1700014N06Rik |
| Chromosome | X |
| Deletion Start | 133,461,906 |
| Deletion End | 133,462,824 |
| Deletion Size | 919 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,462,210 | 133,462,229 | AGCAGGAGAAGCCTGAGGTG |
| TUP | 133,462,230 | 133,462,251 | CAGCGGCGTCGCTCACTTCACC |
| TUR | 133,461,314 | 133,461,333 | AGACGGGCTCTACGCTCATC |
| TDF | 133,462,374 | 133,462,397 | GTGAACATCCTGGATTCATTCGTG |
| TDP | 133,462,405 | 133,462,433 | TGTTTGAAAGAATTGCATCCGAAGCCAGC |
| TDR | 133,462,443 | 133,462,467 | TGATAGTAGAGTTTCTGGCTTGACG |
PCR Primers
| Primer | Sequence |
| SU | CTCAGACAGGTATCTTCCTC |
| SD | GGCCAAAATTTCCACAAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 916 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12100A-B7 | VGB6 | C57BL/6NTac | |
| 12100A-E9 | VGB6 | C57BL/6NTac | |
| 12100A-G7 | VGB6 | C57BL/6NTac | |
