12104 Nr2f2 Status: ES cell colonies screened / QC no positives
| Gene | Nr2f2 |
| Chromosome | 7 |
| Deletion Start | 70,364,058 |
| Deletion End | 70,358,376 |
| Deletion Size | 5,683 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,359,808 | 70,359,826 | TAGCCTGCTCTGGGTAAGG |
| TUP | 70,359,776 | 70,359,797 | CCCGAGCTCTCCCGGTTGCAGT |
| TUR | 70,359,748 | 70,359,769 | CCCGGTTCCTAGTACCAAATCC |
| TDF | 70,355,243 | 70,355,264 | TGATGGTTGGGTTCTGTTTGTC |
| TDP | 70,355,210 | 70,355,237 | ACTTTGCACCAATACACTGTCTTAGGCC |
| TDR | 70,355,169 | 70,355,189 | CAGGCTTTCATGGCTCCAGTC |
PCR Primers
| Primer | Sequence |
| SU | CAGACAAGCAATCGACAAAAC |
| SD | GTTGTTCTGCCCAACACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
