| Gene | Alkbh4 |
| Chromosome | 5 |
| Deletion Start | 136,139,479 |
| Deletion End | 136,141,336 |
| Deletion Size | 1,858 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,140,142 | 136,140,162 | TGGCCCTAACTGCTTCTCCTT |
| TUP | 136,140,165 | 136,140,188 | TGTCTCCACTGCAGGACTATGGCC |
| TUR | 136,140,215 | 136,140,234 | TGGAAGCCTGCCATCTTCAG |
| TDF | 136,141,049 | 136,141,068 | TGTTTCCAGCCAAGGATGAC |
| TDP | 136,141,071 | 136,141,095 | TGAACTCCGGATCCTCCTGCCTCTA |
| TDR | 136,141,099 | 136,141,118 | TGGCAATCCCAGCATTCAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGCTATTGTGGGTCTTTGC |
| SD | AAAGCGAGTTCCAAGACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12106A-B9 | VGB6 | C57BL/6NTac | |
| 12106A-E11 | VGB6 | C57BL/6NTac | |
| 12106A-F7 | VGB6 | C57BL/6NTac | |
| 12106A-A3 | VGB6 | C57BL/6NTac | |
| 12106A-A4 | VGB6 | C57BL/6NTac | |
