12117 Lrmp Status: ES cell colonies screened / QC no positives
| Gene | Lrmp |
| Chromosome | 6 |
| Deletion Start | 145,137,540 |
| Deletion End | 145,174,681 |
| Deletion Size | 37,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,138,238 | 145,138,261 | GAATGAACGAGGACCAGAGTACAG |
| TUP | 145,138,263 | 145,138,292 | CGTGAGTACGGACAGAGATCTGTCTTGTTC |
| TUR | 145,138,316 | 145,138,337 | GAAGGAGGAACAGCCCTCAAAG |
| TDF | 145,173,947 | 145,173,967 | TTTACACCAGGCAGGAACTTG |
| TDP | 145,173,968 | 145,173,989 | ACCTCGGTCCAGGCAGCCTTGG |
| TDR | 145,174,010 | 145,174,027 | GCTGCTGAAGCCAGAGAC |
PCR Primers
| Primer | Sequence |
| SU | ACCTCTATGTAGCTAAGCATC |
| SD | TCCAGCCACACTGCACAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
